| Products/Services Used | Details | Operation |
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| Gene Synthesis | Genes encoding the LPMOs were commercially synthesised and codon optimised by GenScript, USA. Codon-optimised gene sequences for NcLPMO9E and NcLPMO9F are shown in Fig. S1 and S2, ESI,† respectively. The synthetic genes encoded native secretion signals at the N-terminus and (His)6 tags at the C-terminus of the corresponding proteins. The genes were inserted into the EcoRI-NotI position of the P. pastoris expression vector pPICZB, in which the c-myc epitope and polyhistidine tag were omitted. The constructs were transformed into E. coli TOP10 cells (Thermo Fisher Scientific) by electroporation, and the transformants were cultivated in low salt Luria–Bertani (LB) broth containing 25 μg mL−1 zeocin (Invitrogen). | Get A Quote |
The production of cellulose nanofibres (CNFs) typically requires harsh chemistry and strong mechanical fibrillation, both of which have negative environmental impacts. A possible solution is offered by lytic polysaccharide monooxygenases (LPMOs), oxidative enzymes that boost cellulose fibrillation. Although the role of LPMOs in oxidative modification of cellulosic substrates is rather well established, their use in the production of cellulose nanomaterials is not fully explored, and the effect of the carbohydrate-binding module (CBM) on nanofibrillation has not yet been reported. Herein, we studied the activity of two LPMOs, one of which was appended to a CBM, on delignified softwood fibres for green and energy... More
