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Gene Synthesis> | A synthetic and codon optimized gene of a NCBI genbank entry XM_003663599 was ordered from GenScript (New Jersey, USA). The gene was ordered to be cloned via HindIII and XhoI in a pET28a vector with kanamycin resistance such that an Nterminal 6x-Histidine tag was translationally fused to CYP505A30. Plasmid integrity was confirmed by sequencing (GATC-Biotech, Germany). | Get A Quote |
Among nature’s arsenal of oxidative enzymes, cytochrome P450s (CYPs) catalyze the most challenging reactions, the hydroxylations of non-activated CH bonds. Human CYPs are studied in drug development due to their physiological role at the forefront of metabolic detoxification, but their challenging handling makes them unsuitable for application. CYPs have a great potential for biocatalysis, but often lack appropriate features such as high and soluble expression, self-sufficient internal electron transport, high stability, and an engineerable substrate scope. We have probed these characteristics for a recently described CYP that originates from the thermophilic fungus Thermothelomyces thermophila (CYP505A30), a... More