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Gene Synthesis> | cDNAs of human IRF5 variants, V5, V1, V2/V6, and V3/V4, were developed and used for retroviral gene transfer to create HA-tagged or FLAG-tagged IRF5 V5, V1, V2/V6 or V3/V4 MEFs as previously described (76). N-terminal HA-tagged eIF4G1 plasmids were generated using pcDNA3 vector as previously described (77). FLAG-eIF4A and FLAG-PABP expression vectors were obtained from GenScript. Detailed information is provided in the Supplemental Experimental Procedures. | Get A Quote |
Interferon Regulatory Factor 5 (IRF5) plays an important role in limiting pathogenic infection and tumor development. Host protection by IRF5 can occur through a variety of mechanisms including production of type I interferon and cytokines as well as the regulation of cell survival, growth, proliferation, and differentiation. While modulation of these cellular processes is attributed to IRF5 transcription factor function in the nucleus, emerging evidence suggests that IRF5 may also retain non-transcriptional regulatory properties within the cytoplasmic compartment. Consistent with this notion, we report the ability of IRF5 to control gene expression at the level of mRNA translation. Our findings demonstrate tha... More