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Gene Synthesis> | The coding regions for full-length enzymes containing an N-terminal 6-histidine purification tag were order as designer genes from Genscript (Piscataway, NJ, USA), and the proteins were purified from conditioned media from transfected HEK293-EBNA cells on Ni-chelating IMAC agarose (Qiagen, Hilden, Germany). | Get A Quote |
Mast cells (MC) are resident tissue cells found primarily at the interphase between tissues and the environment. These evolutionary old cells store large amounts of proteases within cytoplasmic granules, and one of the most abundant of these proteases is tryptase. To look deeper into the question of their in vivo targets, we have analyzed the activity of the human MC tryptase on 69 different human cytokines and chemokines, and the activity of the mouse tryptase (mMCP-6) on 56 mouse cytokines and chemokines. These enzymes were found to be remarkably restrictive in their cleavage of these potential targets. Only five were efficiently cleaved by the human tryptase: TSLP, IL-21, MCP3, MIP-3b, and eotaxin. This stri... More