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Gene Synthesis> | The genes coding for the SP of R. baltica (Genbank/EMBL/DDBJ accession number KF733603) and P. amoebophila (YP_008052) were synthesized (GenScript Corp. USA) flanked by restriction sites for EcoRI (Thermo Scientific) and XhoI (Thermo Scientific) that were used for subsequent cloning into pGEX 4T-1 vector (GE Healthcare) containing an N-terminal GST tag at the multiple cloning site. | Get A Quote |
The phyla Planctomycetes, Verrucomicrobia, Chlamydiae, Lentisphaerae, and "Candidatus Omnitrophica (OP3)" comprise bacteria that share an ancestor but show highly diverse biological and ecological features. Together, they constitute the PVC superphylum. Using large-scale comparative genome sequence analysis, we identified a protein uniquely shared among all of the known members of the PVC superphylum. We provide evidence that this signature protein is expressed by representative members of the PVC superphylum. Its predicted structure, physicochemical characteristics, and overexpression in Escherichia coli and gel retardation assays with purified signature protein suggest a housekeeping function with unspecific ... More