| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | The scFv constructs were designed with VH and VL separated by a flexible linker (G4S)3, codon-optimized, synthesized by Genscript and cloned into a promoter-modified pcDNA Hygro (+)-CMV+vector58 with a immunoglobulin κ chain leader sequence and a C-terminal S-peptide tag followed by a hexa-histidine tag. | Get A Quote |
Nipah virus (NiV) and Hendra virus (HeV) are zoonotic henipaviruses (HNVs) responsible for outbreaks of encephalitis and respiratory illness with fatality rates of 50-100%. No vaccines or licensed therapeutics currently exist to protect humans against NiV or HeV. HNVs enter host cells by fusing the viral and cellular membranes via the concerted action of the attachment (G) and fusion (F) glycoproteins, the main targets of the humoral immune response. Here, we describe the isolation and humanization of a potent monoclonal antibody cross-neutralizing NiV and HeV. Cryo-electron microscopy, triggering and fusion studies show the antibody binds to a prefusion-specific quaternary epitope, conserved in NiV F and HeV F... More
