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Molecular Biology Tools> | Protein mass prediction programs (available at https://www.genscript.com/tools/peptide-molecular-weight-calculator as well as https://proteomics.cf.gu.se/proteomics/tools/peptide-calculator) gave an average molecular mass of monomeric disulphide bonded protein with N-terminal methionine and a hexa-histidine tag as 11.963 kDa and mono-isotopic mass of 11.960 kDa which are very close to the observed mass. | Get A Quote |
Cyanovirin-N (CV-N), a lectin of cyanobacterial origin, has important medical implications due to its highly effective virucidal activity. However, its low production yield has limited its application till date. In this study, we demonstrate for the first time, a simple process for a break-through production of soluble and bioactive recombinant CV-N (rCV-N) in a newer strain called Escherichia coli SHuffle® T7 Express lysY that is engineered for enhanced production of correctly disulphide bonded proteins. As rCV-N contains two critical disulphide bonds required for its anti-HIV activity, rational choice of this expression host could produce high yield of soluble rCV-N (∼24 mg/ 100 ml) in simple Erlenmeyer ... More