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Molecular Biology Reagents> | An E. coli codon-optimized version of VC1 coding for an N-terminal His-tag and lacking the predicted cTP-coding 446 region (Supplementary File 7) was synthesized (GenScript) and cloned into expression vector pET22b(+) 447 using restriction sites NdeI and HindIII. | Get A Quote |
Faba bean is a widely adapted and high-yielding legume cultivated for its protein-rich seeds1. However, the seeds accumulate the anti-nutritional pyrimidine glucosides vicine and convicine, which can cause haemolytic anaemia—favism—in the 400 million individuals genetically predisposed by a deficiency in glucose-6-phosphate dehydrogenase2. Here, we identify the first enzyme associated with vicine and convicine biosynthesis, which we name VC1. We show that VC1 co-locates with the major QTL for vicine and convicine content and that the expression of VC1 correlates highly with vicine content across tissues. We also show that low-vicine varieties express a version of VC1 carrying a small, frame-shift inse... More