| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The allele genes joined with the identical CDR3 and FR4 (ARLRDGFNNGFDYWGQGTLVTVSS), as well as a previously reported camelid-derived nanobody that targets human TNF-α (12), were synthesized (Genscript, Nanjing, China), and then subcloned into the pComb3x vector with N-terminal OmpA signal peptide (MKKTAIAIAVALAGFATVAQA) and C-terminal tags comprising a hexahistidine (His6) tag and a Flag tag in tandem. | Get A Quote |
The worldwide spread of COVID-19 highlights the need for an efficient approach to rapidly develop therapeutics and prophylactics against SARS-CoV-2. The SARS-CoV-2 spike protein, containing the receptor-binding domain (RBD) and S1 subunit involved in receptor engagement, is a potential therapeutic target. We describe the development of a phage-displayed single-domain antibody library by grafting naive complementarity-determining regions (CDRs) into framework regions of a human germline immunoglobulin heavy chain variable region (IGHV) allele. Panning this library against SARS-CoV-2 RBD and S1 subunit identified fully human single-domain antibodies targeting five distinct epitopes on SARS-CoV-2 RBD with subnanom... More
