| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | To create the expression vector for the gp46-I(162-214), the DNA encoding gp46-I(162-214) was optimized according to E. coli codon usage by JAVA codon optimization tool (http://www.jcat.de/).Rare codon analysis tools (https://www.genscript.com/tools/rare-codon-analysis) were used to analyze the codons; they were then synthesized by Biomatik (Wilmington, USA). The synthetic gene was sub-cloned into the BamHI/EcoRI sites of the pGS21a vector using standard protocols. The gp46-I(162-214) fused with a histidine tag (His-tag) and a GST tag; and enterokinase cleavage site at the N-terminus was expressed from the resulting plasmid, which is referred to as pGS21a-gp46-I(162-214). | Get A Quote |
Background: Gp46-I(162-214) is a linear peptide derived from human T-lymphotropic virus Type 1 (HTLV-1) gp46-I envelope protein. This protein is commonly used to detect HTLV-1 antibodies during enzyme-linked immunosorbent assay (ELISA) or Western blotting. Objectives: This study reported a simple and efficient method for large-scale preparation of this peptide, which is employed for diagnostic purposes. Methods: This study was a descriptive research. The DNA encoding gp46-I(162-214) was optimized according to E. coli codon usage. It was then synthesized and sub cloned into a pGS21a vector. This vector added a His-GST tag to the N-terminus of the protein. The pGS21a-gp46-I(162-214) was transformed into the che... More
