至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Targeted polyubiquitylation of RASSF1C by the Mule and SCFbeta-TrCP ligases in response to DNA damage.

Biochem J.. 2012-01;  441(1):227-36
Xin Zhou, Ting?Ting Li, Xu Feng, Esther Hsiang, Yue Xiong, Kun?Liang Guan and Qun?Ying Lei. Molecular and Cell Biology Lab, Institute of Biomedical Sciences, Fudan University, Shanghai 200032, China; School of Life Sciences, Fudan University, Shanghai 200032, China
Products/Services Used Details Operation

摘要

RASSF1A [Ras association (RalGDS/AF-6) domain family member 1A] and RASSF1C are two ubiquitously expressed isoforms of the RASSF1 gene. The promoter of RASSF1A is frequently hypermethylated, resulting in inactivation in various human cancers. RASSF1A is implicated in the regulation of apoptosis, microtubule stability and cell cycle arrest. However, little is known about the regulation and function of RASSF1C. In the present study we show that exogenously expressed RASSF1C is a very unstable protein that is highly polyubiquitylated and degraded via the proteasome. Furthermore, RASSF1C degradation is enhanced when cells are exposed to stress signals, such as UV irradiation. Mule, a HECT (homologous with E6-associ... More

关键词

DNA damage; glycogen synthase kinase 3 (GSK3); phosphoinositide 3-kinase (PI3K); stability; Ras association (RalGDS/AF-6) domain family member 1C (RASSF1C); ubiquitylation.