Products/Services Used | Details | Operation |
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Gene Synthesis> | ...A bicistronic construct encoding His-tagged RelB and untagged RelE-R81A mutant was synthesized (<b>GenScript</b>) and inserted into plasmid pMG25 (Christensen-Dalsgaard et al., 2008)... | Get A Quote |
SummaryTranslational control is widely used to adjust gene expression levels. During the stringent response in bacteria, mRNA is degraded on the ribosome by the ribosome-dependent endonuclease, RelE. The molecular basis for recognition of the ribosome and mRNA by RelE and the mechanism of cleavage are unknown. Here, we present crystal structures of E. coli RelE in isolation (2.5 Å) and bound to programmed Thermus thermophilus 70S ribosomes before (3.3 Å) and after (3.6 Å) cleavage. RelE occupies the A site and causes cleavage of mRNA after the second nucleotide of the codon by reorienting and activating the mRNA for 2′-OH-induced hydrolysis. Stacking of A site codon bases with conse... More