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Custom Vector Construction> | … Materials and Methods. Creation of Recombinant N‐CEACAMs 4, 5, 6, and 8. Coding sequences for the recombinant expression of N‐CEACAM were synthesized and subcloned into the pGEX‐2T vector (containing a glutathione S‐transferase (GST)‐tag) by GenScript … | Get A Quote |
Human carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) are a family of receptors that mediate intercellular interactions. Pathogenic bacteria have ligands that bind CEACAMs on human cells. Neisseria gonorrhoeae (Gc) encodes numerous unique outer membrane opacity-associated (Opa) proteins that are ligands for one or more CEACAMs. CEACAMs that are expressed on epithelial cells facilitate Gc colonization, while those expressed on neutrophils affect phagocytosis and consequent intracellular survival of Gc. Since Opa protein expression is phase-variable, variations in receptor tropism affect how individual bacteria within a population interact with host cells. Here we report the development of a ra... More