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Mammalian Expression> | … 2.2 Plasmids, mammalian cells, and transfections. Ataxin‐3 and eGFP variants were synthesized by the company Genscript (genscript.com) and subcloned into HA‐tagged, pCDNA3.1(+1) for ataxin‐3 or pCDNA3.1+N‐eGFP for eGFP … | Get A Quote |
Ataxin-3 is a deubiquitinase and polyglutamine disease protein whose cellular properties and functions are not entirely understood. Mutations in ataxin-3 cause spinocerebellar ataxia type 3 (SCA3), a neurodegenerative disorder that is a member of the polyglutamine family of diseases. Two major isoforms arise from alternative splicing of ATXN3 and are differently toxic in vivo as a result of faster proteasomal degradation of one isoform compared to the other. The isoforms vary only at their C-termini, suggesting that the hydrophobic C-terminus of the more quickly degraded form of ataxin-3 (here referred to as isoform 2) functions as a degron-that is, a peptide sequence that expedites the degradation of its host ... More