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Custom Vector Construction> | … In the FATT solubility purification, FATT was synthesized by GenScript © and cloned into the … ii. FATT Vector Construction The FATT sequence was synthesized by GenScript © into a pUC57 vector using NdeI and SacI. This sequence can be seen as Figure 9 … | Get A Quote |
High throughput methods for recombinant protein production using E. coli typically involve the use of affinity tags for simple purification of the protein of interest. One drawback of these techniques is the occasional need for tag removal before study, which can be hard to predict. In this work, we demonstrate two high throughput purification methods for untagged protein targets based on simple and cost-effective self-cleaving intein tags. Two model proteins, E. coli beta-galactosidase (βGal) and superfolder green fluorescent protein (sfGFP), were purified using self-cleaving versions of the conventional chitin-binding domain (CBD) affinity tag and the nonchromatographic elastin-like-polypeptide (ELP) preci... More