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Custom Vector Construction> | … MG1655 was synthesized (GenScript, USA) and subcloned into the IPTG (Isopropyl β‐D‐1‐Thiogalactopyranoside)‐inducible pMCSG7 vector with a short N‐terminal 6‐His tag without TEV cleavage site.45 This truncated protein was prepared at the Recombinant Protein … | Get A Quote |
RcsB, the transcription-associated response regulator of the Rcs phosphorelay two-component signal transduction system, activates cell stress responses associated with desiccation, cell wall biosynthesis, cell division, virulence, biofilm formation, and antibiotic resistance in enteric bacterial pathogens. RcsB belongs to the FixJ/NarL family of transcriptional regulators, which are characterized by a highly conserved C-terminal DNA-binding domain. The N-terminal domain of RcsB belongs to the family of two-component receiver domains. This receiver domain contains the phosphoacceptor site and participates in RcsB dimer formation; it also contributes to dimer formation with other transcription factor partners. He... More