Products/Services Used | Details | Operation |
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GenParts™ DNA Fragments> | … expression vector. A DNA fragment (1590 bp, Fig. S2) including the ORF of BsCat (1575 bp), KpnI and XhoI restriction enzyme sites and a 6×His-tag coding sequence was synthesized by GenScript Corporation (Nanjing, China) … | Get A Quote |
In this study, the catalase gene of Chinese black sleeper Bostrychus sinensis (termed as BsCat) was sequenced and characterized. The BsCat, which encodes 525 amino acids, contains a catalase proximal active site signature domain (FDRERIPERVVHAKGAG) and a catalase proximal heme-ligand signature domain (RLFAYPDTH). The BsCat exhibits high sequence similarity with Cat of other species. Phylogenetic tree reconstruction revealed a close evolutionary relationship of BsCat to catalase genes of other fishes. The results of Real-time PCR showed that the BsCat gene was constitutively expressed in most organs of B. sinensis, with predominant expression detected in liver, followed by peripheral blood and spleen. Moreover, ... More