Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction> | … Invitrogen. All other chemicals were from Sigma-Aldrich unless otherwise indicated. Flag-tagged TRMT6 and TRMT61A mammalian expression vectors were purchased from Genscript. Two additional Flag tags where inserted into the vector coding TRMT61A … | Get A Quote |
Post-transcriptional methylation of N6- and N1-adenine can effect transcriptome turnover and translation. Furthermore, the regulatory function of N6-methyladenine (m6A) during heat shock has been uncovered, including the enhancement of the phase separation potential of RNAs. In response to acute stress, e.g. heat shock, the orderly sequestration of mRNAs in stress granules (SGs) is considered important to protect transcripts from the irreversible aggregation. Until recently, the role of N1-methyladenine (m1A) on mRNAs during acute stress response remains largely unknown. Here we show that the methyltransferase complex TRMT6/61A, which generates the m1A tag, is involved in transcriptome protection during heat sh... More