| Products/Services Used | Details | Operation |
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| Proteins, Expression, Isolation and Analysis | … SSAT) led to an increase in DiAcSpm in MCF-7 human breast carcinoma cells [3]. The N 1 -acetylated derivatives of spermidine … for 15 min, GST-tagged proteins were purified from the supernatant by affinity chromatography on 0.3 mL of glutathione resin (GenScript Japan, Tokyo … | Get A Quote |
We developed a new procedure for the comprehensive analysis of metabolites and enzymes involved in polyamine metabolism pathways. The procedure utilizes stable isotope-labeled polyamines and directly and precisely determines labeled products from enzymatic reactions by ESI-Q-TOF-MS. The activity of different enzymes could be determined in essentially the same manner by suitably adjusting the reaction conditions for each individual enzyme. We applied the procedure to extracts of regenerating rat liver and analyzed the changes in polyamine-metabolizing enzymes and polyamine contents during recovery from partial hepatectomy. A general outline of polyamine metabolism and information of polyamine dynamics were obtai... More
