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Proteins, Expression, Isolation and Analysis> | … Lysates were pre-cleared by centrifugation at 20,000 g for 10 min and supernatants were incubated with 40 μl anti-Flag Affinity Resin (GenScript, Nanjing, China) for 5 h at 4°C before washing three times with ice-cold wash buffer (IBA BioTAGnology) … | Get A Quote |
Influenza A virus (IAV) causes seasonal infections and periodic pandemics in humans. The non-structural protein 1 (NS1) of IAV is the main viral antagonist of the innate immune responses that play a key role in influenza pathogenesis. However, the mechanism to disrupt the host cell homeostasis by IAV NS1 remains poorly understood. Here, we show that expression of NS1 from the WSN strain, but not PR8 strain, of IAV, markedly induced nuclear import of the host RNA interference (RNAi) factors such as Argonaute-2 and microRNA 16. We found that the single residue substitution of aspartic acid with histidine at position 101 (D101H) of IAV-PR8 NS1 was sufficient to induce the nuclear import process and to enhance the ... More