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Recombinant Antibody Expression> | … Purification of the recombinant glucosyltransferase UGTSL2 was performed with the AKTA Primer Plus (GE Healthcare Bio-science, Uppsala, Sweden) and a high affinity Ni-charged resin FF prepacked column (GenScript, Nanjing, China), since a 6-histidine tag was added at … | Get A Quote |
Rebaudioside E, one of the minor components of steviol glycosides, was first isolated and identified from Stevia rebaudiana in 1977. It is a high-intensity sweetener that tastes about 150-200 times sweeter than sucrose and is also a precursor for biosynthesis of rebaudioside D and rebaudioside M, the next-generation Stevia sweeteners. In this work, new unknown steviol glycosides were enzymatically synthesized from stevioside by coupling UDP-glucosyltransferase UGTSL2 from Solanum lycopersicum and sucrose synthase StSUS1 from Solanum tuberosum. Rebaudioside E was speculated to be the main product of glucosylation of the Glc(β1→C-19) residue of stevioside along with the formation of a (β1→2) linkage based o... More