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Bacterial Expression> | … the original procedure described by Wu et al. (2012). The E. coli codon-optimized gene for Arabidopsis UVR8 (see Methods S2 for the gene sequence) was introduced into the pET11a expression vector generating a construct carrying an N-terminal 6×His-tag (Genscript). The … | Get A Quote |
The photoreceptors UV RESISTANCE LOCUS 8 (UVR8) and CRYPTOCHROMES 1 and 2 (CRYs) play major roles in the perception of UV-B (280-315 nm) and UV-A/blue radiation (315-500 nm), respectively. However, it is poorly understood how they function in sunlight. The roles of UVR8 and CRYs were assessed in a factorial experiment with Arabidopsis thaliana wild-type and photoreceptor mutants exposed to sunlight for 6 or 12 hr under five types of filters with cut-offs in UV and blue-light regions. Transcriptome-wide responses triggered by UV-B and UV-A wavelengths shorter than 350 nm (UV-A ) required UVR8 whereas those induced by blue and UV-A wavelengths longer than 350 nm (UV-A ) required CRYs. UVR8 modulated gen... More