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Monoclonal Antibody Services> | … Then primary antibody against VSV-G protein (VSV-G-tag Antibody, pAb, Rabbit, source: GenScript) or dystrophin (monoclonal anti-dystrophin, clone MANDYS8) was added at a dilution of 1:100 in 2.5% BSA-DPBS for one and half hours. Cells were again washed in DPBS … | Get A Quote |
Duchenne muscular dystrophy (DMD) and mdx mouse dystrophy result from mutations in the dystrophin gene. Although these mutations are primarily responsible for the defects that underlie the pathology of dystrophinopathies, other factors may contribute importantly to the pathology. In the present investigation, we tested whether T cells present in mdx muscles are activated and contribute significantly to the pathological process. Flow cytometric analyses showed a significantly higher frequency of activated CD44(high) T cells in the blood and muscle of mdx mice when compared to normal B10 mice. However, the frequency of activated T cells was not elevated in mdx lymph nodes, suggesting muscle-specific T cell activa... More