Products/Services Used | Details | Operation |
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GenParts™ DNA Fragments> | … the TCF binding motifs of the M50 Super 8x TOPflash reporter (a gift from Randall Moon, Addgene plasmid # 12,456) with pre-designed dsDNA fragments (GeneArt Strings, Thermo Fisher Scientific, Waltham, USA; or custom clones, GenScript, Piscataway, USA) containing … | Get A Quote |
Forkhead box (FOX) family transcription factors play essential roles in development, tissue homeostasis, and disease. Although the biology of several FOX proteins has been studied in depth, it is unclear to what extent these findings apply to even closely related family members, which frequently exert overlapping but non-redundant functions. To help address this question, we have generated a uniform, ready-to-use expression library of all 44 human FOX transcription factors with a convenient peptide tag for parallel screening assays. In addition, we have generated multiple universal forkhead box reporter plasmids, which can be used to monitor the transcriptional activity of most FOX proteins with high fidelity. ... More