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Plasmid DNA Preparation> | … 4 | MATERIALS AND METHODS 4.1 | Protein expression and purification. cDNA sequences encoding the protein variants were purchased from GenScript and included an N-terminal tag (6xHis-Lipo domain). Plasmids encoding the protein constructs … | Get A Quote |
The interaction between the transcriptional coactivators CREBBP/p300 and NCOA is governed by two intrinsically disordered domains called NCBD and CID, respectively. The CID domain emerged within the NCOA protein in deuterostome animals (including vertebrates) after their split from the protostomes (molluscs, worms, and arthropods). However, it has not been clear at which point a high affinity interaction evolved within the deuterostome clade and whether all present-day deuterostome animals have a high affinity NCBD:CID interaction. We have here expressed and measured affinity for NCBD and CID domains from animal species representing different evolutionary branches of the deuterostome tree. While all vertebrate ... More