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Plasmid DNA Preparation> | … The plasmid for the expression of cjFetM-PD with a thrombin-cleavable N-terminal polyHis-tag was ordered from GenScript. The fetM-PD gene was inserted into pET15b using NdeI and BamHI. Expression of P19 and FetP. Production … | Get A Quote |
Campylobacter jejuni is a leading cause of food-borne gastrointestinal disease in humans and uropathogenic Escherichia coli is a leading cause of urinary tract infections. Both human pathogens harbour a homologous iron uptake system (termed cjFetM-P19 in C. jejuni and ecFetM-FetP in E. coli). Although these systems are important for growth under iron limitation, the mechanisms by which these systems function during iron transport remain undefined. The copper ions bound to P19 and FetP, the homologous periplasmic proteins, are coordinated in an uncommon penta-dentate manner involving a Met-Glu-His3 motif and exhibit positional plasticity. Here we demonstrate the function of the Met and Glu residues in modulating... More