Products/Services Used | Details | Operation |
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Monoclonal Antibody Services> | … After incubating at 18°C for 2 h followed by three times of washing, 100 μl mouse anti-His tag monoclonal antibody (Genscript, China) diluted to 1:2,000 was added and incubated at 37°C for 1 h. The plate was washed again, and 100 μl of rabbit-anti-mouse Ig-AP conjugate … | Get A Quote |
Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oyster is, however, yet to be discovered. This work makes contributions by identifying CD63H, a CD63 homolog with four transmembrane domains and one conservative CCG motif, and establishing its role as a receptor that participates in immune recognition and hemocyte phagocytosis. The presence of CD63H messenger RNA (mRNA) in hepatopancreas, labial palps, gill, and hemocytes is confirmed. The expression level of mRNA in hemocytes is found significantly ( < 0.01) upregulated after ... More