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Plasmid DNA Preparation> | … N-terminal c-myc-tagged caMC4R, N-terminal FLAG-tagged caMRAP2a, and N-terminal FLAG-tagged caMRAP2b were synthesized and subcloned into pcDNA3.1 vector by GenScript (Piscataway, NJ, USA) to obtain the expression plasmids … | Get A Quote |
Melanocortin-4 receptor (MC4R) plays important roles in regulation of multiple physiological processes, and interaction of MC4R and melanocortin receptor accessory protein 2 (MRAP2) is suggested to play pivotal role in energy balance of vertebrates. Topmouth culter () is an economically important freshwater fish in China. Herein we cloned culter , and . Culter consisted of a 981 bp open reading frame encoding a protein of 326 amino acids. qRT-PCR revealed that , and were primarily expressed in the central nervous system. In the periphery, and were expressed more widely in the male, while was expressed more widely in the female. Culter MC4R could bind to four peptide agonists and increase intracellular cAMP... More