Products/Services Used | Details | Operation |
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Custom DNA/RNA Oligos> | … The following custom oligopeptides (Fig 1) were from the following suppliers: KASASHHHHHH, KACAK, and KAKAK from GenScript; and SAAAK(biotin) (with biotin covalently attached to the ε-amino group of lysine), biotin-AAAS (with biotin covalently attached to the N-terminus … | Get A Quote |
Investigation of the biological roles of inorganic polyphosphate has been facilitated by our previous development of a carbodiimide-based method for covalently coupling primary amine-containing molecules to the terminal phosphates of polyphosphate. We now extend that approach by optimizing the reaction conditions and using readily available "bridging molecules" containing a primary amine and an additional reactive moiety, including another primary amine, a thiol or a click chemistry reagent such as dibenzocyclooctyne. This two-step labeling method is used to covalently attach commercially available derivatives of biotin, peptide epitope tags, and fluorescent dyes to the terminal phosphates of polyphosphate. Add... More