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Plasmid DNA Preparation> | … The 3A102 VH gene was synthesized by the Invitrogen company (Genscript, USA) and re-cloned into the pET-28b vector (Genscript, USA) to construct a 6xHis-tag fused 3A102 VH recombinant gene. After that, the 3A102 recombinant plasmid was transformed into E. coli Shuffle … | Get A Quote |
Alpha folate receptor (FRα) is currently under investigation as a target for the treatment of patients with non-small-cell lung cancer (NSCLC), since it is highly expressed in tumor cells but is largely absent in normal tissue. In this study, a novel human variable domain of a heavy-chain (VH) antibody fragment specific to FRα was enriched and selected by phage bio-planning. The positive phage clone (3A102 VH) specifically bound to FRα and also cross-reacted with FRβ, as tested by ELISA. Clone 3A102 VH was then successfully expressed as a soluble protein in an shuffle strain. The obtained soluble 3A102 VH demonstrated a high affinity for FRα with affinity constants (K) values around 7.77 ± 0.25 ×... More