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Catalog Peptides> | … A synthetic peptide of residues 940–961 of Fcp1 with an N-terminal FITC-Ahx fluorescent tag was purchased from GenScript, dissolved in phosphate buffered saline, and stored in 1 mM aliquots at −80 C. This construct, which we termed f-Fcp1x, was designed to contain the … | Get A Quote |
Intrinsically disordered protein (IDP) sequences often contain a high proportion of charged residues in conjunction with their high degree of hydrophilicity and solvation. For high net charge IDPs, long-range electrostatic interactions are thought to play a role in modulating the strength or kinetics of protein-protein interactions. In this work, we examined intramolecular interactions mediated by charged regions of a model IDP, the C-terminal tail of the phosphatase Fcp1. Specifically, this work focuses on intermolecular interactions between acidic and basic patches in the primary structure of Fcp1 and their contributions to binding its predominantly basic partner, the winged helix domain of Rap74. We observe ... More