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Catalog Peptides> | … coli K12 cells containing a genome FLAG tag on the UbiF gene (GenScript, Piscataway, NJ, USA) were cultured in the LEX bioreactor (Epiphyte3 Inc., Toronto, Canada) for 24 h at 37 C in the M9 medium with 0.5% succinate as the sole carbon source, yielding approx. 3 g of … | Get A Quote |
The pyruvate dehydrogenase complex (PDHc) links glycolysis to the citric acid cycle by converting pyruvate into acetyl-coenzyme A. PDHc encompasses three enzymatically active subunits, namely pyruvate dehydrogenase, dihydrolipoyl transacetylase, and dihydrolipoyl dehydrogenase. Dihydrolipoyl transacetylase is a multidomain protein comprising a varying number of lipoyl domains, a peripheral subunit-binding domain, and a catalytic domain. It forms the structural core of the complex, provides binding sites for the other enzymes, and shuffles reaction intermediates between the active sites through covalently bound lipoyl domains. The molecular mechanism by which this shuttling occurs has remained elusive. Here, we ... More