Products/Services Used | Details | Operation |
---|---|---|
Mutant Libraries> | … The genes were ordered from GenScript Inc The RBD construct contains the SAS- COV-2 spike protein (residues 319–591), followed by a GGGGS linker and an 8XHis tag in a pcDNA3.4 modified vector (SI). Its mutants, including RBDN501Y, RBDK417N, RBDE484K, and … | Get A Quote |
SARS-CoV-2 has been spreading around the world for the past year. Recently, several variants such as B.1.1.7 (alpha), B.1.351 (beta), and P.1 (gamma), which share a key mutation N501Y on the receptor-binding domain (RBD), appear to be more infectious to humans. To understand the underlying mechanism, we used a cell surface-binding assay, a kinetics study, a single-molecule technique, and a computational method to investigate the interaction between these RBD (mutations) and ACE2. Remarkably, RBD with the N501Y mutation exhibited a considerably stronger interaction, with a faster association rate and a slower dissociation rate. Atomic force microscopy (AFM)-based single-molecule force microscopy (SMFS) consisten... More