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Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies

Access Microbiol. 2021-08;

Inna Sekirov, Martin Petric, Erin Carruthers, David Lawrence, Tamara Pidduck, Jesse Kustra, Jonathan Laley, Min-Kuang Lee, Navdeep Chahil, Annie Mak, Paul N Levett, Emelissa Mendoza, Heidi Wood, Mike Drebot, Mel Krajden, Muhammad Morshed

Products/Services Used	Details	Operation
Recombinant Proteins	… Surrogate virus microneutralization test (sVNT). sVNT tests (GenScript USA, Inc., NJ, USA) were performed following the manufacturer's instructions … Discussion. We compared the GenScript sVNT assay to cVNT, PRNT and commercial CLIA assays …	Get A Quote

摘要

We compared neutralization assays using either the wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus or surrogate neutralization markers, using characterized sera. We found the results of the neutralization assays 75 % concordant overall and 80 % concordant for samples with high antibody levels. This demonstrates that commercial surrogate SARS-CoV-2 assays offer the potential to assess anti-SARS-CoV-2 antibodies' neutralizing capacity outside CL-3 laboratory containment.