Products/Services Used | Details | Operation |
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Recombinant Antibody Expression> | … Mouse Anti-cGFP-tag mAb antibodies were purchased from GenScript. … The purified recombinant protein was used in liquid chromatography–tandem mass spectrometry (LC–MS/MS) conducted by Sangon Biotech. All the primers used are listed in Table S1. … | Get A Quote |
Genetic manipulation of plant genes in prokaryotes has been widely used in molecular biology, but the function of a DNA sequence is far from being fully known. Here, we discovered that a plant protein-coding gene containing the CRAL_TRIO domain serves as a promoter in bacteria. We firstly characterized CitPITP1 from Citrus, which contains the CRAL_TRIO domain, and identified a 64-bp sequence (key64) that is critical for prokaryotic promoter activity. In vitro experiments indicated that the bacterial RNA polymerase subunit RpoD specifically binds to key64. We then expanded our research to fungi, plant and animal species to identify key64-like sequences. Five such prokaryotic promoters were isolated from Amborell... More