Products/Services Used | Details | Operation |
---|---|---|
Plasmid DNA Preparation> | … A synthetic construct encoding His-tagged SUMO-AXH-C (Ataxin-1 residues 563–816) from the pCDFDuet-1 plasmid was ordered from Genscript. The plasmid was transformed by heat shock at 42 C to NiCo21(DE3) cells. A single colony was used to inoculate 30 mL 2TY … | Get A Quote |
Expansion of the polyglutamine tract in the N terminus of Ataxin-1 is the main cause of the neurodegenerative disease, spinocerebellar ataxia type 1 (SCA1). However, the C-terminal part of the protein - including its AXH domain and a phosphorylation on residue serine 776 - also plays a crucial role in disease development. This phosphorylation event is known to be crucial for the interaction of Ataxin-1 with the 14-3-3 adaptor proteins and has been shown to indirectly contribute to Ataxin-1 stability. Here we show that 14-3-3 also has a direct anti-aggregation or "chaperone" effect on Ataxin-1. Furthermore, we provide structural and biophysical information revealing how phosphorylated S776 in the intrinsically d... More