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Molecular Biology Reagents> | … RBD protein expressed with AviTag was purchased from GenScript. Site-specific biotinylation of the AviTag was performed using BirA Biotin-Protein Ligase Reaction kit (Avidity). Next, unconjugated biotin was removed using Zeba spin desalting columns, 7K MWCO (… | Get A Quote |
The COVID-19 pandemic underscores the need for rapid, safe, and effective vaccines. In contrast to some traditional vaccines, nanoparticle-based subunit vaccines are particularly efficient in trafficking antigens to lymph nodes, where they induce potent immune cell activation. Here, we developed a strategy to decorate the surface of oxidation-sensitive polymersomes with multiple copies of the SARS-CoV-2 spike protein receptor-binding domain (RBD) to mimic the physical form of a virus particle. We evaluated the vaccination efficacy of these surface-decorated polymersomes (RBD) in mice compared to RBD-encapsulated polymersomes (RBD) and unformulated RBD (RBD), using monophosphoryl-lipid-A-encapsulated polymersome... More