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DNA Sequencing> | … ApoL1-R150A was constructed by DNA synthesis using the ApoL1 sequence (RefSeq “G4,” NM_003661, with E150/M228/R255) with an N-terminal flag-TEV cleavage sequence tag as the template (GenScript Biotech). The protein was expressed in SF9 insect cells and purified … | Get A Quote |
Apolipoprotein L1 (ApoL1) is a circulating innate immunity protein protecting against trypanosome infection. However, two ApoL1 coding variants are associated with a highly increased risk of chronic kidney disease. Here we present X-ray and NMR structures of the N-terminal domain (NTD) of ApoL1 and of its closest relative ApoL2. In both proteins, four of the five NTD helices form a four-helix core structure which is different from the classical four-helix bundle and from the pore-forming domain of colicin A. The reactivity with a conformation-specific antibody and structural models predict that this four-helix motif is also present in the NTDs of ApoL3 and ApoL4, suggesting related functions within the small Ap... More