Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction> | … The pET28a(+) vectors containing DNA encoding for Gx-SB, Gx-LB, calibrator luciferase (mNG-NL), and SUMO-TNFα-SB were ordered from GenScript. The sequence of calibrator luciferase was reported by Suzuki et al. , and a His-tag at the N-terminus and a Strep-tag at the C-… | Get A Quote |
Heterogeneous immunoassays such as ELISA have become indispensable in modern bioanalysis, yet translation into point-of-care assays is hindered by their dependence on external calibration and multiple washing and incubation steps. Here, we introduce RAPPID (Ratiometric Plug-and-Play Immunodiagnostics), a mix-and-measure homogeneous immunoassay platform that combines highly specific antibody-based detection with a ratiometric bioluminescent readout. The concept entails analyte-induced complementation of split NanoLuc luciferase fragments, photoconjugated to an antibody sandwich pair via protein G adapters. Introduction of a calibrator luciferase provides a robust ratiometric signal that allows direct in-sample c... More