| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | … Briefly, T2 cells were incubated with a concentration series of the test peptide (GenScript) in AIM V serum-free medium (Thermo Fisher Scientific) for 1 hour at 37C. The cells were then transferred to a lower temperature (26C) for another 14 hours, before returning them to 37C … | Get A Quote |
A central feature of the SARS-CoV-2 pandemic is that some individuals become severely ill or die, whereas others have only a mild disease course or are asymptomatic. Here we report development of an improved multimeric αβ T cell staining reagent platform, with each maxi-ferritin "spheromer" displaying 12 peptide-MHC complexes. Spheromers stain specific T cells more efficiently than peptide-MHC tetramers and capture a broader portion of the sequence repertoire for a given peptide-MHC. Analyzing the response in unexposed individuals, we find that T cells recognizing peptides conserved amongst coronaviruses are more abundant and tend to have a "memory" phenotype, compared to those unique to SARS-CoV-2. Significa... More
