Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | … The pET-19b plasmid that contained the EryB gene labelled with C-terminal 6×His-Tag was designed by GenScript Biotech. The plasmid was transformed into Escherichia coli BL21(DE3) competent cells. The cells were then grown in LB Amp medium at 37 C. … | Get A Quote |
Ostreolysin A6 (OlyA6) is a protein produced by the oyster mushroom (). It binds to membrane sphingomyelin/cholesterol domains, and together with its protein partner, pleurotolysin B (PlyB), it forms 13-meric transmembrane pore complexes. Further, OlyA6 binds 1000 times more strongly to the insect-specific membrane sphingolipid, ceramide phosphoethanolamine (CPE). In concert with PlyB, OlyA6 has potent and selective insecticidal activity against the western corn rootworm. We analysed the histological alterations of the midgut wall columnar epithelium of western corn rootworm larvae fed with OlyA6/PlyB, which showed vacuolisation of the cell cytoplasm, swelling of the apical cell surface into the gut lumen, and ... More