| Products/Services Used | Details | Operation |
|---|---|---|
| Molecular Biology Reagents | … sVNT kit was purchased from Genscript and the assay was performed according to the manufacturer’s instructions 38 . Briefly, diluted serum samples were mixed with HRP-RBD at a 1:1 ratio and incubated at 37 C for 30 min. An aliquot (100 µL) of the mixture was moved to a … | Get A Quote |
Better diagnostic tools are needed to combat the ongoing COVID-19 pandemic. Here, to meet this urgent demand, we report a homogeneous immunoassay to detect IgG antibodies against SARS-CoV-2. This serological assay, called SATiN, is based on a tri-part Nanoluciferase (tNLuc) approach, in which the spike protein of SARS-CoV-2 and protein G, fused respectively to two different tNLuc tags, are used as antibody probes. Target engagement of the probes allows reconstitution of a functional luciferase in the presence of the third tNLuc component. The assay is performed directly in the liquid phase of patient sera and enables rapid, quantitative and low-cost detection. We show that SATiN has a similar sensitivity to ELI... More
