| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | … DNA for the Homo sapiens NKCC1 and Mus musculus KCC2 were obtained from GenScript and cloned into the PEG BacMam vector for virus amplification and protein purification. A twin-Strep affinity tag was inserted after the C terminus of NKCC1, and the same tag was … | Get A Quote |
NKCC and KCC transporters mediate coupled transport of Na+K+Cl and K+Cl across the plasma membrane, thus regulating cell Cl concentration and cell volume and playing critical roles in transepithelial salt and water transport and in neuronal excitability. The function of these transporters has been intensively studied, but a mechanistic understanding has awaited structural studies of the transporters. Here, we present the cryo-electron microscopy (cryo-EM) structures of the two neuronal cation-chloride cotransporters human NKCC1 (SLC12A2) and mouse KCC2 (SLC12A5), along with computational analysis and functional characterization. These structures highlight essential residues in ion transport and allow us to prop... More
