Products/Services Used | Details | Operation |
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Monoclonal Antibody Services> | … Ultra-15 centrifugal filter tube equipped with Ultracel-10 membrane was made by Amicon (USA). Mouse anti-His6 monoclonal antibody and horseradish peroxidase conjugated anti-mouse IgG antibody were obtained from GenScript (Nanjing, China) … | Get A Quote |
Tobacco etch virus protease (TEVp) is an enzymatic reagent to remove fusion tag, but additional purification steps are required for removing the TEVp after cleavage reaction is finished. Use of carrier-free and dependent TEVp immobilizates can eliminate protease contamination. In this work, we identified that, among the four constructed missense variants, the insoluble variant with the highest activity was correspondent with the soluble one tested formerly. The activities of the insoluble 15 codon variants were assayed and the variant with highest activity was selected. The K45F and/or E106G mutations have been reported on slightly improving protein stability of the wild-type TEVp, but only E106G mutation enhan... More