| Products/Services Used | Details | Operation |
|---|---|---|
| DNA Sequencing | … reaction (PCR) amplification (Simon et al. 2006). Then PCR products were sequenced using primer-walking strategy from both strands by Genscript Biotech Corp. (Nanjing, China). The mitochondrial genome was assembled by … | Get A Quote |
The complete mitochondrial genome of (Orthoptera: Acrididae: Calliptaminae) from Qinghai Lake, Qinghai province, China is a circular molecule of 15,668 bp in size, and contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and one AT-rich region. The overall nucleotide composition is 41.8% of A, 30.9% of T, 11.3% of G, and 16.0% of C. All PCGs started with typical ATN codon, e.g. one with ATA, two with ATT and ATC, and eight with ATG. Eleven PCGs ended with complete stop codon TAA, and the other two genes (ND1 and ND4L) ended with TAG. Phylogenetic trees were reconstructed with 13 PCGs using Bayesian Inference (BI) and maximum likelihood (ML) to validate the taxonomic status of , exh... More
