Products/Services Used | Details | Operation |
---|---|---|
Monoclonal Antibody Services> | … Mouse monoclonal antibodies were: anti-Flag epitope (M2, Sigma), anti-IP 3 R3 #610313 (for immunoblot) and anti-Drp1 #611112 (BD Biosciences), anti-V5 epitope tag (GenScript), anti-Mcl-1 #RC13 (for IP) and anti-streptavidin #S10D4 (ThermoFisher), and anti-p97 … | Get A Quote |
The function of the Bcl-2 family member Bok is currently enigmatic, with various disparate roles reported, including mediation of apoptosis, regulation of mitochondrial morphology, binding to inositol 1,4,5-trisphosphate receptors, and regulation of uridine metabolism. To better define the roles of Bok, we examined its interactome using TurboID-mediated proximity labeling in HeLa cells, in which Bok knock-out leads to mitochondrial fragmentation and Bok overexpression leads to apoptosis. Labeling with TurboID-Bok revealed that Bok was proximal to a wide array of proteins, particularly those involved in mitochondrial fission (e.g., Drp1), endoplasmic reticulum-plasma membrane junctions (e.g., Stim1), and surpris... More