Products/Services Used | Details | Operation |
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ORF cDNA Clones/MolecularCloud> | A mouse CaSR construct with a C-terminal FLAG-tag in pcDNA3.1+ expression vector was purchased from GenScript (ORF clone:OMu14241D) and validated by sequencing (ACGT). | Get A Quote |
Transfer of information across membranes is fundamental to the function of all organisms and is primarily initiated by transmembrane receptors. For many receptors, how ligand sensitivity is fine-tuned and how disease associated mutations modulate receptor conformation to allosterically affect receptor sensitivity are unknown. Here we map the activation of the calcium-sensing receptor (CaSR) - a dimeric class C G protein-coupled receptor (GPCR) and responsible for maintaining extracellular calcium in vertebrates. We show that CaSR undergoes unique conformational rearrangements compared to other class C GPCRs owing to specific structural features. Moreover, by analyzing disease associated mutations, we uncover a ... More