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Mammalian Expression> | Receptor blocking assay A soluble extracellular cadherin repeat 1 (sEC1, GenBank: NM_002587, residues 1 to 172) construct was synthesized and cloned into the pCDNA3.1(+) mammalian cell expression vector by GenScript with a C-terminal GSG linker and decahistidine tag. | Get A Quote |
New World hantaviruses (NWHs) are endemic in North and South America and cause hantavirus cardiopulmonary syndrome (HCPS), with a case fatality rate of up to 40%. Knowledge of the natural humoral immune response to NWH infection is limited. Here, we describe human monoclonal antibodies (mAbs) isolated from individuals previously infected with Sin Nombre virus (SNV) or Andes virus (ANDV). Most SNV-reactive antibodies show broad recognition and cross-neutralization of both New and Old World hantaviruses, while many ANDV-reactive antibodies show activity for ANDV only. mAbs ANDV-44 and SNV-53 compete for binding to a distinct site on the ANDV surface glycoprotein and show potently neutralizing activity to New and ... More