| Products/Services Used | Details | Operation |
|---|---|---|
| Monoclonal Antibody Services | When the OD600 of the broth reaches 0.3–0.5, the inducer SppIP [18] (GenScript Biotech, China) was added into the culture at a concentration of 50 ng/mL...The membrane was blocked by 5% skim milk solution at room temperature for 2 h, followed by incubating with anti-HA tag rabbit polyclonal antibody (1:1000, Proteintech, USA) or anti-RBD mouse monoclonal antibody 13E10D5 (1:1000, GenScript Biotech, China) at 4 °C overnight and washed with TBST four times...The RBD protein expressed by the Chinese hamster ovary (CHO) cell (GenScript Biotech, China) was utilized as standard at 31.25 ng, 62.5 ng, 125 ng, 250 ng, and 500 ng amounts | Get A Quote |
Coronavirus Disease 2019 (COVID-19) caused by a novel betacoronavirus SARS-CoV-2 has been an ongoing global pandemic. Several vaccines have been developed to control the COVID-19, but the potential effectiveness of the mucosal vaccine remains to be documented. In this study, we constructed a recombinant L. plantarum LP18:RBD expressing the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein via the surface anchoring route. The amount of the RBD protein was maximally expressed under the culture condition with 200 ng/mL of inducer at 33 °C for 6 h. Further, we evaluated the immune response in mice via the intranasal administration of LP18:RBD. The results showed that the LP18:RBD significantly elici... More
