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Mutant Libraries> | The expression of unique components of the PC (GP129, GP131, and GP133) by GP83dPC+ mutant virus was verified by Western blot of infected cell lysate (ectopic GP129 myc tagged protein, Figure 1D) or immunofluorescence of GP83dPC+ virus infected GPL fibroblast cells with custom antibodies (Genscript) [25] for GP131 (Figure 1I,J | Get A Quote |
A vaccine against congenital cytomegalovirus infection is a high priority. Guinea pig cytomegalovirus (GPCMV) is the only congenital CMV small animal model. GPCMV encodes essential glycoprotein complexes for virus entry (gB, gH/gL/gO, gM/gN) including a pentamer complex (gH/gL/GP129/GP131/GP133 or PC) for endocytic cell entry. The cohorts for protection against congenital CMV are poorly defined. Neutralizing antibodies to the viral glycoprotein complexes are potentially more important than an immunodominant T-cell response to the pp65 protein. In GPCMV, GP83 (pp65 homolog) is an evasion factor, and the GP83 mutant GPCMV has increased sensitivity to type I interferon. Although GP83 induces a cell-mediated respon... More